Intervalos de coletas de sêmen em reprodutores suínos no início da vida reprodutiva: avaliação dos parâmetros seminais
Dornelles, JulianniRossi, Carlos Augusto RigonSchlösser, Luara Medianeira de LimaRech, Rodrigo DalminaGräf, Cristian GuilhermeAndrade, Cínthia Melazzo deSoares, MarceloCeron, Marcos SperoniPatias, Jovani
Background: In swine production, good reproduction rates can be achieved through genetic selection and reproductivebiotechnologies. One of these biotechnologies is artificial insemination, which contributes to disseminate genes and optimizebreeding boars, thus improving the quality of insemination doses. This study focused on evaluating the intervals betweensemen collection from boars at the beginning of their reproductive maturity vis-à-vis the viability of insemination doses.Materials, Methods & Results: Twenty 9-month-old boars of the genetic lineage AGPIC 337 (Agroceres PIC) were used inthis study. The experimental design used here was completely randomized, and the randomly selected males were dividedinto four treatment groups, which were named according to the interval between semen sample collections: T1: 2 days; T2:3 days; T3: 4 days and T4: 7 days. Each treatment comprised 5 animals, and at the end of the 90 days of this study, a totalof 150 ejaculates were obtained in T1, 110 in T2, 90 in T3 and 60 in T4. The values of total motility, volume and spermconcentration of the ejaculates were evaluated, as was oxidative stress by means of thiobarbituric acid reactive substances(TBARS), the latter after 0, 72, 120 and 168 h of the study. Membrane integrity was evaluated at 0, 72, 120 and 168 h usingthe eosin-nigrosin staining procedure. Sperm heat resistance was tested after 120 h, and sperm morphology after 72, 120 and168 h. Sperm concentrations differed, with T3 showing 27.04% and 29.65% higher concentrations (P < 0.05) than groupsT2 and T1, respectively. Total motility in group T4 was 0.56%, 1.98% and 3.28% higher (P < 0.05) than in T3, T2 and T1,respectively, indicating that the 7-day interval produced the best result. The heat...(AU)
Texto completo