Ocorrência de hemogregarina em duas espécies de cágados brasileiros

Pessoa, Lourdes Marina BezerraScalon, Marcela CorrêaCarneiro, Filipe TavaresLima, Maíra Gonçalves da MotaSilva, Thamiris Figueiredo daMonteiro, Rafael VeríssimoPaludo, Giane Regina

Background: Hemogregarines are the most common intraerythrocytic parasites found in reptiles. The genus Haemogregarina has aquatic vertebrates as intermediate hosts, and as definitive hosts the leeches. The genus Hepatozoon can be found parasitizing amphibians, reptiles, birds and mammals and its main vectors invertebrates are mosquitoes, tsetse flies, lice, fleas and mites. The diagnosis of these parasites is done by the technique of blood smear, but modern diagnoses include evaluation of blood by polymerase chain reaction (PCR). The aim this study was to determine the occurrence of infection by hemogregarine in freshwater turtles, through PCR. Materials, Methods & Results: Samples from 99 freshwater turtles of species P. expansa and P. geoffroanus of Fundação Zoológico de Brasília,Distrito Federal, Brazil, were used. The animals was captured using a hand net, and were immediately individually identified. The blood samples was collected by puncture of the occipital sinus, and placed into tubes containing sodium heparin anticoagulant for hematologic and molecular analysis. Two different sets of oligonucleotides were used, one to detect hemogregarines and other to detect Hepatozoon sp. infection. The presence of hemogregarine was detected in 20 samples analyzed (n = 99), these eleven samples were positive for hemogregarine, 5 were Hepatozoon sp. and 4 were positive for both [...](AU)

1. Hepatozoon
2. PCR
3. Hemoparasites
4. Freshwater turtles
5. Hemogregarine