Associação entre fatores de virulência e resistência antimicrobiana de Escherichia coli enterotoxigênicas isoladas de leitões com diarreia no Brasil
Sato, José Paulo HirojiTakeuti, Karine LudwigDaniel, Amanda Gabrielle de SouzaKoerich, Priscila Karina VitorBernardi, Mari LourdesBarcellos, David Emilio Santos Neves de
Background: Enterotoxigenic Escherichia coli (ETEC) is the etiologic agent of post weaning colibacillosis, one of the most important diseases in pig farming. The pathogenesis of the disease is associated with two virulence factors (VF), fimbriae and enterotoxins. In veterinary medicine, the use of antibiotics can lead to the selection of resistant bacteria. The association of VF and antibiotic resistance is an important mechanism for bacterial survival under adverse conditions. This study aimed to determine the VF and antimicrobial susceptibility of ETEC isolates from piglets with diarrhea and analyze the association between these factors.Materials, Methods & Results: A total of 185 rectal swabs were collected from weaned piglets in Brazilian farms of the states of Rio Grande do Sul, Santa Catarina, Paraná, Minas Gerais and Goiás. The isolation of ETEC was carried out on blood and MacConkey Agar and characterization by biochemical tests and detection by PCR of fimbrial genes F4, F45, F6, F18 and F41, and toxins genes LT, STa, STb and STx2e. Antimicrobial susceptibility profiles were determined by Agar diffusion test for amoxicillin, ampicillin, ceftiofur, ciprofloxacin, colistin, doxycycline, enrofloxacin, florfenicol, gentamicin, neomycin, norfloxacin, oxytetracycline, streptomycin, tetracycline, lincomycin + spectinomycin and sulfamethoxazole + trimethoprim. The association between VF and antimicrobials resistance results was determined by Chi-square and Fisher test (P ≤ 0.05). A total of 376 isolates were analyzed. The frequencies of fimbriae and toxins amplified were: F4 (31.6%), F18 (18.9%), F5 (4.2%) and toxins STa (43.1%), STb (24.7%), LT (21.8%) and STx2e (5.3%). Antibiotic resistance was higher to tetracycline (96.3%), florfenicol (95.2%), oxytetracycline (93.62%) and doxycycline (90.7%). Lowest levels of resistance were to ceftiofur (2.1%), colistin (9.8%), lincomycin + spectinomicin (15.4%) and neomycin (23.1%).[...](AU)
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