Influência das épocas do ano na morfometria testicular e epididimária, características do sêmen e proteínas do sêmen em SDS-PAGE em zebus e taurinos
Chacur, Marcelo George MungaiReis, Juliana Domingos Archanjo dosTavares, Lucas SilvaSanches, KlismanGuaberto, LucianaAlves, Vagner CamariniOba, EuniceRamos, Alcides Amorim
Background: Seminal plasma is a set of secretions from accessory male reproduction organs which infl uence spermaticfunctions. Climate changes that affect gametogenesis may produce low reproduction effi ciency in bulls. Electrophoresisis a great help in the diagnosis of reproduction pathologies and in animal differentiation with regard to fertility within thecontext of climate changes. Current research aims at studying the infl uence of the rainy and dry periods in testicle and epididymis morphometry, semen characteristics and protein of seminal plasma by SDS-PAGE electrophoresis in extensivelybred Nellore and Simental bulls.Materials, Methods & Results: Five Nellore and two Simental bulls, at mean age of four years, were employed. Semencollection was undertaken during the rainy (spring and summer) and dry (autumn and winter) at 15-day intervals. Onehundred and fi fty-four ejaculations were analyzed and scrotal, testicle and epididymis measurement was provided. Samplesof semen plasma were centrifuged and conditioned at -196°C till electrophoresis processing. Proteins were extracted from200 µL from each sample in an extraction buffer composed of 0.625 M Tris - HCl, pH 6.8; 2% SDS, 5% β - mercaptoethanol and 20% glycerol. Proteins were quantifi ed and electrophoreses processed. Data were evaluated by analysis ofvariance and means compared by Tukey´s test at 5%. The expression TV = 0.0396 x (average testicle length) x (scrotalperimeter)2 was used for testicle volume (TV), with TV = 460.14 cm3 for the Simental breed during the rainy season andTV = 571.26 cm3 during the dry season. Nellore bulls showed TV = 524.75 cm3 during the rainy season and 515.13 cm3during the dry season. TV increased in Simental bulls during the dry period, whereas Nellore breed increased during therainy one. There was an increase (P < 0.05) in big and total defects during the rainy season...(AU)
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