Immunohistochemial study on effectors of ovary medulla blood vessel in pseudopregnant rabbit
Parillo, FrancescoMaranesi, MargheritaBrecchia, GabrieleGobbetti, AnnaBoiti, CristianoZerani, Massimo
Background: The ovarian blood flow provides oxygen-rich blood and nutrients which are necessary for the growth and secretory activities of follicles and corpora lutea (CL) in cycling and pregnant animals. The blood flow of the ovary is finely regulated by local and systemic mechanisms that integrate nervous, endocrine, and metabolic signals. In the rabbit ovary, administration of prostaglandin F2 (PGF2) to pseudopregnant or pregnant animals affects specific vascular mechanisms regulating luteolytic process, in particular, the regulatory role of progesterone on luteal function through direct and uterine-mediated mechanisms. Thus, the main objective of the present work was to evaluate, at the level of ovarian blood vessels, the immunopresence of the receptors for progesterone (PR-R), PGF2 (FP) and GnRH (GnRH-R), and that of the enzymes endothelial nitric oxide synthase (eNOS), cyclooxygenase 1 (COX1), COX2, and prostaglandin E2-9-ketoreductase (PGE2-9-K).Materials, Methods & Results: Sexually mature New Zealand White female rabbits were used for all experiments. The animals were housed individually, in an indoor facility under controlled conditions of light and temperature, and feed ad libitum. The rabbits were treated with equine chorionic gonadotropin followed 2 days later by an intramuscular injection of a GnRH analogue to induce pseudopregnancy. Five rabbits...(AU)
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