Avaliação in vitro de carrapaticidas no controle de Rhipicephalus (Boophilus) microplus (Canestrini, 1887), no norte do estado do Rio de Janeiro, Brazil
Ferreira Vita, GilmarDumas, EricaAngélica Vieira da Costa Pereira, MariaFerreira, Ildemar
Background: Rhipicephalus (Boophilus) microplus (Canestrini, 1887) traditionally known as cattle tick, is the species that most affects cattle productivity levels in Brazil. The parasite causes annual losses up to US$ 2 billion. Despite several researches carried out all over the globe, it still presents great challenge, especially due to evidence of resistance to chemical products used in plague control. The goal of this project was to verify in vitro efficiency of pesticides in controlling R. (B.) microplus, at Campos dos Goytacazes municipality, Rio de Janeiro, Brazil, in 2007.Materials, Methods & Results: The in vitro test used was the one recommended by FAOs (Food and Agriculture Organization) World Acaricides Resistance Reference Center (WARRC). Engorged females were manually collected off the ground from stalls and separated in two treatments, amitraz 12.5% (commercial dilution 1:500 = 2 mL/1000 mL) and cypermethrin 15% (commercial dilution 1:1000 = 1 mL/1000 mL), each one with six groups of ten parasites. A different solution - 1:10, 1:100, 1:1000, 1:100000 and 1:1000000 - for both acaricides tested was used on each group, and for control was used potable water. The test was started from the highest dilution. Each dilution was repeated three times simultaneously. Recommended dilutions in the research were in the protocol. Each group of engorged females was weighted
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