Evaluation of a latex agglutination kit for detecting rotavirus in piglets
Paesi, SuelenElonir da Rosa, CacianoRodrigues Marocco Dornelles, Phelipeda Luz, FelipeAugusto Schenkel, CesarZampieri, Denise
Background: The group A rotavirus is the most important ethiological agent that causes diarrhea in newborn humans and animals. They belong to the Reoviridae family and have a genome consisting of 11 segments of double-stranded RNA enclosed in a triple-layered capsid. Rotaviruses are classifi ed in six groups (A to G) based on the VP6 capsid protein or on the migration pattern of genomic segments in polyacrylamide gel. Groups A, B, and C were found either in humans or in animals, while groups D to G were found only in animals. The outer layer is formed by two proteins, VP7 and VP4, which elicit neutralizing antibody responses and form the basis of the current dual classifi cation system in G (VP7) and P (VP4) types. The early diagnostic of this viral infection in raising farms is crucial for preventing the dissemination of the disease among the animals. Latex agglutination tests (LAT) are quick and easily done, and require low fi nancial investments.Materials, Methods & Results: The present study evaluated the Rotavirus Latex® kit (Richmond Immunosystems Diagnostics) currently used with humans for detecting the presence of rotavirus antigen in diarrheic feces of piglets. In order to confi rm the results obtained with the LAT, samples were analyzed by EIA with the Ridascreen rotavirus® kit (R-Biopharm). The results were compared with the rotavirus determination test by polyac
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