Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1)

Oliveira, Anna Paula deDavid, Cíntia deEsteves, Paulo AugustoSpilki, Fernando RosadoSilva, Alessandra D’Avila daHolz, CarineSimonetti, Amauri BragaRoehe, Paulo Michel

The method of collection as well as the packaging conditions in which samples are submitted to laboratories play a critical role on the acquisition of reliable results on diagnostic tests. Alternative methods however have been proposed, as the adsorption of blood or serum in filter paper. In this work, it was evaluated the viability of using serum or whole blood samples from bovines collected in filter paper for serological testing against bovine herpesvirus type 1 (BoHV-1).[...](AU)

1. filter paper adsorbed samples
2. bovine herpesvirus
3. indirect ELISA
4. serum neutralization