Effect of Hormonal Supplementation Periods and In Vitro Maturation Media on Developmental Competence of Pig Oocytes

Lima, Alecssandra Sobreira deMarques, Mariana GrokeNascimento, Anibal BallarottiAssumpção, Mayra Elena Ortiz D’AvilaVisitin, José Antonio

The oocyte ability to undergo successful fertilization, cleavage and embryonic development depends on meiotic maturation and developmental competence acquisition. In vitro maturation (IVM) protocols currently use eCG, hCG or a combination of both, the effect of these gonadotrophins during IVM and subsequent embryonic development is still controversial. Several media have been used for IVM of porcine oocytes: TCM199, Whitten’s and NCSU23 have also been shown to support pig oocyte IVM. This study was designed to determine the effect of hormonal supplementation period and maturation media during in vitro maturation of pig oocytes (1) and subsequent embryonic development (2).(AU)

1. hormonal supplementation
2. fertilization
3. swine
4. polispermy, in vitro maturation
5. maturation media