Fungal microbiota isolated from healthy pig skin
Bonfim Carregaro, FabianoSpanamberg, AndréiaMaria Cavallini Sanches, EdnaSiqueira Argenta, JulianaIsabel Brayer Pereira, DanielaZanette, RégisMorais Santurio, JanioEmílio Santos Neves de Barcellos, DavidFerreiro, Laerte
Background: Researches have been developed to observe the normal microbiota of different animal species. This subject is of major importance for the control of potential infection risks. Fungi can be found in various substrates, foodstuffs (cereals, meat, milk, vegetables) and also in the skin, mucosae, respiratory and gastrointestinal tracts of animals. With the dissemination of immunosuppressive diseases in swine herds over the last years, the number of concomitant diseases caused by opportunist microorganisms is gradually increasing in literature. The objective of this study was to determine the microbiota of pig skin with no apparent lesions. Materials, Methods and Results: A number of 261 pigs from 11 swine farms located in six municipalities of the State of Rio Grande do Sul, in Southern Brazil, were used for the study, in the period from April 2005 to April 2006. After being cleaned with water and 70% ethanol, skin samples were collected by friction of circular and sterile hair brushes against the posterior ventral region of the animals, on an area of no more than 10 cm. After sample collection, the brushes were wrapped with the same aluminium foil used in the sterilization process. Within the next 24 hours, the material was streaked onto agar and incubated at 25C to 30ºC for up to four weeks. Micromorphology was used for mold identification purposes, and the process
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