Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats
Schimming, Bruno CesarCirino, Mucio Luiz de AssisLizarte Neto, Fermino SanchesNovais, Paulo CezarCarvalho, Camila Albuquerque Melo deTirapelli, Daniela Pretti da CunhaMolina, Carlos Augusto FernandesTirapelli, Luis FernandoTucci Junior, Silvio
Purpose To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.(AU)
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