Remote ischemic conditioning protects against testicular ischemiareperfusion injury in rats
Damasceno, Ananda Vitória Barros SuzukiBarros, Charles Alberto Villacorta dePercario, SandroRibeiro Junior, Rubens Fernando GonçalvesMonteiro, Andrew MoraesGouveia, Eduardo Henrique HerbsterHenriques, Higor Yuri Bezerra
Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemiareperfusion (IR) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows: Sham, IR, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mgkg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p 0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p 0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.(AU)
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