Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
Campelo, Mariana Barbosa DiasSantos, Joelita de Alencar FonsecaMaia Filho, Antonio Luiz MartinsFerreira, Daniel Cabral LeãoSantAnna, Luciana BarrosOliveira, Rauirys Alencar deMaia, Leonardo FonsecaArisawa, Emilia Ângela Loschiavo
Purpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.Methods: Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.Results: The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).Conclusion: Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.(AU)
Texto completo