Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model

Carvalho, Camila Albuquerque Melo de; Tirapelli, Daniela Pretti da Cunha; Rodrigues, Andressa Romualdo; Lizarte Neto, Fermino Sanches; Novais, Paulo Cézar; Silva, Jairo Pinheiro; Carlotti Júnior, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luís Fernando

p. 629-637

Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model

Carvalho, Camila Albuquerque Melo deTirapelli, Daniela Pretti da CunhaRodrigues, Andressa RomualdoLizarte Neto, Fermino SanchesNovais, Paulo CézarSilva, Jairo PinheiroCarlotti Júnior, Carlos GilbertoColli, Benedicto OscarTirapelli, Luís Fernando

PURPOSE: To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism. METHODS: Fifty Wistar rats were used and divided into: control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study. RESULTS: Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA. CONCLUSION: We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.(AU)