Effects of blocking av b3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats
Oliveira, Claudio Ricardo deMarqueti, Rita de CassiaCominetti, Marcia ReginaDouat, Estela San'Ana VieiraRibeira, Juliana UemaPontes, Carmen Lucia SallaBorghi-Silva, AudreySelistre-de-Araujo, heloisa Sobreiro
PURPOSE: To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats. METHODS: Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-αv β3 antibodies-treated and G4, anti-α2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated. RESULTS: After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti- αv β3 -integrin antibodies produced similar results than DisBa-01 but not anti-α2 integrin blocking antibodies. CONCLUSION: DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia.(AU)
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