Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Ikejiri, Adauto TsutomuSomaio Neto, FredericoChaves, Jose CarlosBertoletto, Paulo RobertoTeruya, RobertoBertoletto, Eduardo RodriguesTaha, Murched OmarFagundes, Djalma Jose

To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm 2.(AU)

1. Mice
2. Gene Expression
3. Lung
4. Oxidative Stress
5. Reperfusion Injury