Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Lessa, Thais BorgesCarvalho, Rafael CardosoSpagnolo, Júlio DavidSilva, Luis Claudio Lopes Correia daCortopassi, Silvia Renata GaidoAmbrósio, Carlos Eduardo

PURPOSE:To investigate the development of a laparoscopy technique for local injection into the X-linked muscular dystrophy (mdx) diaphragm.METHODS:It was used 10 mice Balb/C57 and 5 mdx mice and three differents decubitus type were tested: the right lateral, supine, and supine decubitus with 20 degrees elevation of the forelimb. Abdominal caudal face and the 10 intercostal space were tested as spot to introduce the needle into the diaphragm.RESULTS:Supine position with elevation of 20 degrees forelimb and the 10th intercostal space are the beneficial position to apply a local injection.CONCLUSION:It was proved to be possible to perform the laparoscopy technique in the X-linked muscular dystrophy diaphragm and this requires a specific position and technique during the surgery.(AU)

1. Diaphragm
2. Muscular Dystrophy
3. Mice
4. Laparoscopy
5. Duchenne
6. Injections