Identification of therapeutic target genes with DNA microarray in multiple myeloma cell line treated by IKK beta/NF-kappa B inhibitor
Dou, AixiaWang, ZhilunZhao, JingjieLiu, JunliZheng, Chengyun
PURPOSE:To explore the mechanism of resistance to IKKβ inhibitor in multiple myeloma (MM) cells and uncover novel therapeutic targets for MM.METHODS:We downloaded the microarray data (GSE8476) from GEO (Gene Expression Omnibus) database. The data were derived from the human MM cells lines (L363 cells) treated with IKKβ inhibitor MLN120b (MLN) for eight, 12 and 24 hours. Furthermore, we applied the Search Tool for the Retrieval of Interacting Genes (STRING) and Expression Analysis Systematic Explorer (EASE) database to construct protein-protein interaction networks and identified over-represented pathway among DEGs (differentially expressed genes).RESULTS:We obtained 108 DGEs in 8h vs. 12h group and 101 ones in 8h vs. 24h group. Most of DGEs were found to be involved in biological regulation. The significant pathways were Ig A pathway and the CAMs pathways. In addition, 24 common DGEs were found in the networks of the two groups such as ICAM 3 and SELL.CONCLUSION:Intercellular adhesion molecule 3 and SELL may be potential targets in multiple myeloma treatment in the future.(AU)
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